Accession Number | SPSED0026023 |
Secreted Protein | α-amylase (AmyS) |
Uniprot Accession Number | P06279 |
GenBank Accession Number | AAA22241.1 |
Source Organism of the Secreted Protein | Geobacillus stearothermophilus |
Nucleotide Sequence of the Secreted Protein | Length = 1650 nt GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA |
Amino Acid Sequence of the Secreted Protein | Length = 549 aa MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP |
Expression Host | Bacillus subtilis 1A751 |
Source Organism of the Signal Peptide | Bacillus subtilis |
Source Gene of the Signal Peptide | lytb |
Signal Peptide Sequence | Length = 25 aa MKSCKQLIVCSLAAILLLIPSVSFA |
Type | Sec |
Yield | 593 (U/mL) |
Performance (Current Yield / Highest Yield) | 0.44 |
Charge | 2 |
Charge (N-Region) | 2 |
Hydrophobicity | 1.6 |
Hydrophobicity Plot |
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Hydrophobicity (H-region) | 2.74 |
Signal Peptide Original Protein Sequence | MKSCKQLIVCSLAAILLLIPSVSFAADSNISVKLLNYIGNKSSISLSPTGFYKVTGDNVAVTDRFAGASRYETATLASNSQWKNPNTVILVNRDIFIDALPVIPLAKKLNAPVLFTQPDTLTKTTERQIAKFNPDNILIIGGARSISKDVENKLKSYGAVKRISGKNRYVLSENIAKQMGSYDKAIVVTGRVFQDALAIAPYAAAHGYPILLTEKDKLPDYDLPKQVIIIGSSFSVSDSVENQIKKTSTVQRIPGSTRYELTANIIKQLKLKADKVVMTNGTKYADVLIGASLASKKNSQILFVKQDSVPAAAKSITKDKATYAYDFIGSTSSISAEVENSLADEFYLADGGTYNLKINSGKLNLENIKTYGNSLRIKPENYSTSNRISLDGKQYLGTVNFSIESTKYIRPVNENIPFEDYLKGVIPNEMPASWSLEALKAQTVAARTYSITKTGTTVPDTTAFQVYGGYSWNSNTNKAVEQTKGKVLKYNGSLITAAYSSSNGGYTEASNEVWSSSVPYLIAKKDTKDPQIGWTLTLSKQQLDTKSLDLTKPSSWWSSATETDSARLSGVKNWILKNKETSADSVKIASIDDLSFSGTTQGQRAKTASMKVKYFVKSSTGSYNLSKITTISVPTSELRTMIGATVFKSTYVTVKKDTSKYTISGKGYGHGIGMSQYGAKARAEAGDSYSSILKFYYPGTTLTSY |
Signal Peptide Original DNA Sequence | TTGAAATCTTGCAAACAATTGATAGTGTGTTCACTTGCTGCAATCTTATTATTGATTCCATCAGTTTCTTTTGCAGCAGACTCAAACATCTCAGTTAAATTGTTAAATTATATTGGAAATAAATCTAGCATCAGTCTATCGCCAACAGGATTTTATAAAGTAACTGGAGATAATGTTGCTGTTACGGATCGTTTTGCAGGCGCTTCACGATACGAAACTGCGACTCTTGCATCCAACAGCCAGTGGAAAAATCCTAATACAGTGATATTGGTGAACCGAGATATTTTTATTGATGCATTGCCTGTTATTCCATTGGCGAAAAAACTGAACGCACCAGTTTTATTTACTCAGCCTGACACGTTAACAAAAACTACTGAAAGACAAATTGCTAAGTTTAACCCTGATAATATTTTAATCATTGGGGGAGCGCGAAGCATTTCAAAAGACGTAGAAAACAAGCTGAAATCATATGGCGCTGTGAAAAGGATAAGCGGAAAAAATAGATATGTCTTATCAGAAAATATTGCAAAGCAAATGGGCAGCTATGATAAAGCGATCGTTGTAACCGGAAGAGTATTTCAAGACGCTTTAGCCATTGCTCCATATGCAGCAGCACACGGATACCCTATTTTGCTTACAGAGAAAGACAAGCTTCCGGATTATGATTTGCCAAAACAAGTCATCATTATAGGCAGTTCATTTAGCGTCAGTGATAGTGTAGAAAACCAAATTAAAAAAACTTCAACTGTGCAAAGGATTCCTGGATCAACCAGATATGAACTTACGGCAAATATAATTAAGCAACTAAAGTTGAAGGCTGATAAAGTCGTAATGACGAACGGGACAAAATATGCAGACGTATTGATAGGCGCTTCGCTTGCCTCGAAGAAAAACAGCCAAATTTTATTTGTTAAACAAGATAGCGTTCCGGCTGCGGCTAAAAGTATAACGAAAGATAAAGCCACATATGCATACGACTTTATTGGAAGTACCAGTTCAATTTCTGCTGAAGTTGAGAATTCACTTGCTGATGAATTTTATTTAGCTGACGGTGGCACCTATAATCTAAAAATCAACAGCGGAAAATTAAACCTTGAAAACATCAAAACTTACGGAAACTCCCTAAGAATTAAACCAGAAAACTATTCTACTTCAAACAGAATTAGTCTTGATGGAAAGCAGTATCTTGGCACGGTTAATTTCTCTATTGAGTCTACTAAATATATCCGACCGGTAAATGAGAATATTCCATTTGAGGATTACTTAAAAGGAGTAATTCCGAATGAAATGCCTGCTAGCTGGTCACTTGAGGCTCTTAAAGCACAGACTGTCGCTGCAAGAACCTACTCAATAACAAAAACAGGAACTACTGTACCTGATACAACCGCTTTTCAAGTTTATGGTGGCTACAGTTGGAATTCGAATACCAATAAAGCGGTAGAGCAAACGAAGGGGAAAGTTCTGAAATATAATGGATCTCTTATAACTGCAGCTTATTCTTCCAGTAATGGTGGTTACACAGAAGCTAGTAATGAAGTGTGGTCGTCAAGTGTACCTTACTTGATTGCAAAAAAAGATACAAAGGATCCTCAAATAGGCTGGACGCTTACTTTGTCAAAACAACAGCTAGATACGAAATCTTTGGACTTAACGAAGCCTTCTTCTTGGTGGTCTTCAGCGACTGAAACTGATTCTGCAAGACTTTCCGGAGTTAAGAACTGGATCTTGAAAAATAAAGAAACAAGCGCTGATTCTGTCAAAATTGCAAGTATTGACGATTTAAGCTTTAGTGGAACGACACAAGGGCAGCGTGCAAAAACAGCATCGATGAAAGTGAAGTATTTTGTTAAAAGCAGTACTGGCTCCTATAACTTGAGCAAAATTACAACCATTAGTGTACCAACAAGTGAGTTAAGAACAATGATTGGAGCGACTGTATTTAAAAGTACTTATGTGACTGTAAAAAAAGATACTTCAAAGTACACAATCAGCGGTAAAGGATATGGTCACGGGATCGGAATGAGTCAATATGGAGCAAAAGCAAGAGCAGAAGCTGGAGATTCATATAGTTCAATTTTGAAATTTTACTATCCAGGTACAACTCTGACAAGTTATTGA |
Reference | Jinlan L, Gang F, Huina D, et al. Optimization of thermostable alpha-amylase secretion by screening of optimal singal peptides in Bacillus subtilis[J]. Industrial Microbiology, 2017, 47(01): 17-23. (In Chinese) |
Miscellaneous | - |