SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0009001

Accession Number SPSED0009001
Secreted Protein Alkaline polygalacturonate lyase (PGL)
Uniprot Accession Number -
GenBank Accession Number -
Source Organism of the Secreted Protein Bacillus subtilis WSHB04-02
Nucleotide Sequence of the Secreted Protein Length = 1206 nt
ATGGATGCTGATTTAGGCCACCAGACGTTGGGATCCAATGATGGCTGGGGCGCGTACTCGACCGGCACGACAGGCGGGTCAAAAGCATCGTCCTTAAATGTGTATACCGTCAGCAACAGAAACCAGCTTGTCTCGGCATTAGGGAAGGAAACGAACACAACGCCAAAAATCATTTATATCAAGGGAACGATTGACATGAACGTAGATGACAATCTGAAGCCGCTTGGTCTAAATGACTATAAAGATCCGGAGTATGATTTGGACAAATATTTGAAAGCCTATGATCCTAGCACATGGGGCAAAAAAGAGCCGTCGGGAACACAAGAAGAAGCGAGAGCACGCTCTCAGAAAAACCAAAAAGCACGGGTTATGGTGGATATCCCTGCAAACACGACGATCGTCGGTTCAGGGACTAACGCTAAAGTCGTGGGAGGAAACTTCCAAATCAAGAGTGATAACGTCATTATTCGCAACATTGAATTCCAGGATGCCTATGATTATTTTCCGCAATGGGATCCGACTGACGGAAGCTCAGGAAACTGGAACTCACAATACGACAACATCACGATAAACGGCGGCACACACATCTGGATTGATCACTGTACATTTAACGACGGTTCGCGTCCGGACAGCACATCACCGAAATATTATGGAAGAAAATATCAGCACCATGACGGCCAAACGGATGCGTCCAACGGCGCTAACTATATCACGATGTCCTACAACTATTATCACGATCATGATAAAAGCTCCATTTTCGGATCAAGTGACAGCAAAACCTCCGATGACGGCAAATTAAAAATTACGCTCCATCATAACCGCTATAAAAATATTGTCCAGCGCGCGCCGAGAGTCCGCTTCGGGCAAGTGCACGTATACAACAACTATTATGAAGGAAGCACAAGCTCTTCAAGTTATCCTTTCAGCTATGCATGGGGAATCGGAAAGTCATCTAAAATCTATGCCCAAAACAATGTCATTGACGTACCGGGACTGTCAGCTGCTAAAACGATCAGCGTATTCAGCGGGGGAACGGCTTTATATGACTCCGGCACGTTGCTGAACGGCACACAGATCAACGCATCGGCTGCAAACGGGCTGAGCTCTTCTGTCGGCTGGACGCCGTCTCTGCATGGATCGATTGATGCTTCTGCTAATGTGAAATCAAATGTCATAAATCAAGCGGGTGCGGGTAAATTAAATTAA
Amino Acid Sequence of the Secreted Protein Length = 401 aa
MDADLGHQTLGSNDGWGAYSTGTTGGSKASSLNVYTVSNRNQLVSALGKETNTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDPEYDLDKYLKAYDPSTWGKKEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIKSDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSPKYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVIDVPGLSAAKTISVFSGGTALYDSGTLLNGTQINASAANGLSSSVGWTPSLHGSIDASANVKSNVINQAGAGKLN
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide bpr
Signal Peptide Sequence Length = 30 aa
MRKKTKNRLISSVLSTVVISSLLFPGAAGA
Type Sec
Yield 304.6 (U/ml)
Performance (Current Yield / Highest Yield) 1
Charge 5
Charge (N-Region) 5
Hydrophobicity 0.5
Hydrophobicity Plot
Hydrophobicity (H-region) 1.96
Signal Peptide Original Protein Sequence MRKKTKNRLISSVLSTVVISSLLFPGAAGASSKVTSPSVKKELQSAESIQNKISSSLKKSFKKKEKTTFLIKFKDLANPEKAAKAAVKKAKSKKLSAAKTEYQKRSAVVSSLKVTADESQQDVLKYLNTQKDKGNADQIHSYYVVNGIAVHASKEVMEKVVQFPEVEKVLPNEKRQLFKSSSPFNMKKAQKAIKATDGVEWNVDQIDAPKAWALGYDGTGTVVASIDTGVEWNHPALKEKYRGYNPENPNEPENEMNWYDAVAGEASPYDDLAHGTHVTGTMVGSEPDGTNQIGVAPGAKWIAVKAFSEDGGTDADILEAGEWVLAPKDAEGNPHPEMAPDVVNNSWGGGSGLDEWYRDMVNAWRAADIFPEFSAGNTDLFIPGGPGSIANPANYPESFATGATDINKKLADFSLQGPSPYDEIKPEISAPGVNIRSSVPGQTYEDGWDGTSMAGPHVSAVAALLKQANASLSVDEMEDILTSTAEPLTDSTFPDSPNNGYGHGLVNAFDAVSAVTDGLGKAEGQVSVEGDDQEPPVYQHEKVTEAYEGGSLPLTLTAEDNVSVTSVKLSYKLDQGEWTEITAKRISGDHLKGTYQAEIPDIKGTKLSYKWMIHDFGGHVVSSDVYDVTVKPSITAGYKQDFETAPGGWVASGTNNNWEWGVPSTGPNTAASGEKVYGTNLTGNYANSANMNLVMPPIKAPDSGSLFLQFKSWHNLEDDFDYGYVFVLPEGEKNWEQAGVYNGKTSSWTDEEIDLSAYKGQNIQVMFNLQSDESIAKEGWYIDDVVLSDKSAGKTVKKNKLGVEKPSGKQKKKPVNPKKAKPSANTAVKHQNKAIQPQVLPLKAQVSVVETGKSTYSDQSTGQYTLKHKAGDYTLMAEAYGYQSKTQKVSLKTDQTTQANFTLEEMKKGTLKGTVINKTTGEPVTGASVYVVEDAAVEPAMTNDKGEYMLEAYEGAYTIKVAAPGYYSDEFSVELKGDVTKETALKPFVGYPGEIAYDDGTAENANSYFAAGNGWAVKMTLADGKDKGMLTGGLFRFWDTEFPDPGGTEFKVEVYDATGKDGAPGKKIAGPFNAEALRNGEWTKVDLSSKGIMVDKDFYLVYIQSKPDPYSPGLAMDETGQNSGRNWQYIDGKWQPGDKADGNYMIRALVDYEAAVPEITSPTDKSYTNKDSVTVKGNASPGTTVHIYNGEKEAGETKAAADGTFHAGIILNKGENELTATASTDNGTTDASSPITVTLDQEKPELTLDNPKDGGKTNKETLTVKGAVSDDNLKDVKVNGKKATVADGSYSARILLENGRNEIKVIATDLAGNKTTKKTVIDVNFDKPVISGLIPGEDKNLKAGESVKIAFSSAEDLDATFTIRMPLTNARASVQNATELPLREISPGRYEGYWTATSSIKAKGAKVEVIVRDDYGNETRKTANGKLNMNTEN
Signal Peptide Original DNA Sequence ATGAGGAAAAAAACGAAAAACAGACTCATCAGCTCTGTTTTAAGTACAGTTGTCATCAGTTCACTGCTGTTTCCGGGAGCAGCCGGGGCAAGCAGTAAAGTCACCTCACCTTCTGTTAAAAAGGAGCTTCAATCTGCGGAATCCATTCAAAACAAGATTTCGAGTTCATTAAAGAAAAGCTTTAAAAAGAAAGAAAAAACGACTTTTCTGATTAAATTTAAAGATCTGGCTAACCCAGAAAAAGCGGCAAAAGCGGCTGTTAAAAAAGCGAAATCGAAGAAGCTGTCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCTGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGCAAGATGTCCTAAAATACTTGAACACCCAGAAAGATAAAGGAAATGCAGACCAAATTCATTCTTATTATGTGGTGAACGGGATTGCTGTTCATGCCTCAAAAGAGGTTATGGAAAAAGTGGTGCAGTTTCCCGAAGTGGAAAAGGTGCTTCCTAATGAGAAACGGCAGCTTTTTAAGTCATCCTCCCCATTTAATATGAAAAAAGCACAGAAAGCTATTAAAGCAACTGACGGTGTGGAATGGAATGTAGACCAAATCGATGCCCCAAAAGCTTGGGCACTTGGATATGATGGAACTGGCACGGTTGTTGCGTCCATTGATACCGGGGTGGAATGGAATCATCCGGCATTAAAAGAGAAATATCGCGGATATAATCCGGAAAATCCTAATGAGCCTGAAAATGAAATGAACTGGTATGATGCCGTAGCAGGCGAGGCAAGCCCTTATGATGATTTGGCTCATGGAACCCACGTGACAGGCACGATGGTGGGCTCTGAACCTGATGGAACAAATCAAATCGGTGTAGCACCTGGCGCAAAATGGATTGCTGTTAAAGCGTTCTCTGAAGATGGCGGCACTGATGCTGACATTTTGGAAGCTGGTGAATGGGTTTTAGCACCAAAGGACGCGGAAGGAAATCCCCACCCGGAAATGGCTCCTGATGTTGTCAATAACTCATGGGGAGGGGGCTCTGGACTTGATGAATGGTACAGAGACATGGTCAATGCCTGGCGTGCGGCCGATATTTTCCCTGAGTTTTCAGCGGGGAATACGGATCTCTTTATTCCCGGCGGGCCTGGTTCTATCGCAAATCCGGCAAACTATCCAGAATCGTTTGCAACTGGAGCGACTGATATCAATAAAAAGCTCGCTGACTTTTCTCTTCAAGGGCCATCTCCATATGATGAAATAAAGCCGGAAATATCTGCACCGGGCGTTAATATTCGTTCATCCGTTCCCGGTCAGACATATGAGGATGGTTGGGACGGCACATCAATGGCAGGGCCGCATGTATCCGCTGTTGCTGCACTGCTGAAACAGGCGAATGCCTCACTTTCTGTTGATGAGATGGAGGATATATTAACCAGCACGGCTGAACCGCTCACGGATTCAACATTTCCTGATTCACCGAATAACGGATATGGCCATGGTCTGGTGAATGCTTTTGATGCTGTATCCGCTGTTACAGATGGATTAGGGAAAGCGGAAGGACAAGTTTCTGTAGAGGGGGATGACCAAGAGCCTCCTGTCTATCAGCATGAGAAAGTAACTGAAGCTTATGAAGGTGGCAGCCTACCACTGACTTTGACAGCTGAAGACAATGTGAGTGTGACATCTGTAAAGCTGTCCTACAAGCTTGATCAAGGTGAATGGACAGAAATAACGGCTAAACGAATCAGCGGTGATCATCTAAAAGGAACGTATCAGGCAGAGATCCCAGATATAAAAGGAACTAAACTAAGCTATAAGTGGATGATTCACGATTTTGGCGGTCATGTCGTTTCGTCTGACGTATACGATGTAACAGTGAAACCAAGCATCACGGCGGGATATAAGCAGGACTTTGAAACTGCACCCGGCGGCTGGGTTGCGAGCGGAACAAATAATAACTGGGAATGGGGAGTTCCGTCAACTGGCCCAAATACAGCAGCATCCGGAGAAAAAGTATATGGAACGAATTTGACAGGAAATTATGCCAACTCAGCAAACATGAACCTTGTTATGCCTCCTATTAAAGCACCTGATTCAGGAAGTCTGTTCCTTCAATTTAAAAGCTGGCACAATTTAGAGGATGATTTTGATTACGGCTACGTTTTTGTTCTTCCGGAAGGTGAAAAGAATTGGGAGCAGGCTGGTGTCTATAACGGAAAGACATCAAGCTGGACGGACGAAGAAATTGATTTATCGGCTTATAAAGGCCAAAACATTCAAGTGATGTTTAACCTTCAATCTGATGAAAGCATTGCAAAAGAGGGCTGGTATATTGATGATGTAGTGCTTTCTGACAAATCAGCCGGAAAAACAGTCAAAAAGAATAAGCTGGGCGTCGAAAAGCCGTCTGGAAAGCAAAAGAAAAAGCCAGTAAATCCGAAAAAGGCTAAGCCATCTGCAAACACAGCGGTAAAACATCAGAACAAGGCTATACAGCCTCAAGTTTTGCCGCTCAAGGCACAAGTCAGTGTAGTGGAAACAGGAAAATCAACATATTCAGATCAATCCACAGGGCAGTACACGCTGAAGCACAAAGCGGGAGACTATACGCTTATGGCAGAAGCATATGGTTATCAGTCGAAAACACAAAAAGTATCTTTAAAGACGGATCAGACGACACAAGCTAATTTTACGTTAGAAGAGATGAAGAAGGGCACATTAAAAGGCACGGTCATCAATAAAACGACAGGAGAGCCGGTAACAGGCGCTTCCGTTTATGTTGTAGAGGATGCTGCGGTGGAACCGGCTATGACAAACGACAAAGGTGAATATATGCTGGAGGCCTATGAAGGCGCTTATACGATTAAAGTCGCTGCACCGGGTTATTACAGTGATGAATTTTCCGTTGAGTTAAAAGGTGATGTTACAAAAGAAACTGCATTGAAGCCTTTCGTCGGTTATCCGGGTGAAATTGCATATGATGACGGAACAGCGGAGAATGCCAATTCCTATTTTGCTGCCGGTAACGGATGGGCGGTAAAAATGACGCTCGCTGACGGCAAGGACAAAGGCATGCTTACAGGAGGGCTGTTCAGATTCTGGGATACAGAGTTCCCTGATCCGGGCGGCACAGAGTTTAAGGTTGAGGTATACGATGCCACAGGAAAAGACGGAGCACCGGGCAAGAAAATTGCAGGGCCATTTAACGCGGAAGCTCTTCGCAATGGCGAGTGGACTAAGGTAGATCTCAGTTCAAAAGGGATTATGGTCGATAAAGATTTTTATCTCGTATATATCCAGTCGAAACCTGATCCGTATTCACCTGGATTGGCAATGGATGAAACCGGCCAGAATTCCGGCCGCAACTGGCAGTATATAGATGGAAAGTGGCAGCCAGGTGACAAAGCGGATGGCAACTATATGATTCGCGCATTAGTTGATTATGAAGCTGCTGTACCTGAGATTACTTCACCGACAGACAAATCATACACAAATAAGGATAGCGTCACTGTAAAAGGAAACGCTTCTCCTGGCACAACGGTACACATTTATAATGGAGAGAAAGAAGCAGGAGAAACGAAAGCTGCTGCGGATGGCACGTTCCATGCAGGCATCATACTCAACAAGGGTGAAAATGAGCTGACGGCAACTGCATCAACTGACAACGGAACAACAGATGCCTCCAGCCCAATCACGGTCACGCTTGATCAAGAAAAGCCTGAATTAACACTGGACAATCCAAAGGATGGCGGGAAAACAAATAAAGAAACGCTGACTGTCAAAGGGGCTGTATCCGATGACAATCTGAAAGACGTCAAGGTGAATGGCAAAAAAGCAACAGTAGCTGATGGTTCATACTCAGCCCGTATTCTTTTGGAAAATGGAAGAAATGAAATCAAGGTAATTGCTACAGACTTGGCAGGCAACAAAACGACGAAAAAGACAGTCATTGATGTGAACTTTGACAAGCCTGTCATTTCCGGCTTAATTCCGGGAGAGGATAAAAACTTAAAAGCCGGTGAATCTGTGAAAATCGCTTTCTCAAGCGCTGAGGATTTAGATGCAACGTTTACCATTCGTATGCCGCTGACCAATGCAAGAGCGAGTGTGCAAAATGCCACCGAACTCCCGTTAAGAGAAATCTCTCCGGGGAGATATGAAGGCTATTGGACTGCCACTTCTTCTATTAAAGCAAAAGGAGCAAAAGTAGAAGTGATCGTCCGAGATGATTATGGAAATGAAACAAGAAAAACTGCGAATGGAAAACTTAATATGAACACAGAAAATTAA
Reference Zhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 [PubMed]
Miscellaneous -