SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0009002

Accession Number SPSED0009002
Secreted Protein Alkaline polygalacturonate lyase (PGL)
Uniprot Accession Number -
GenBank Accession Number -
Source Organism of the Secreted Protein Bacillus subtilis WSHB04-02
Nucleotide Sequence of the Secreted Protein Length = 1206 nt
ATGGATGCTGATTTAGGCCACCAGACGTTGGGATCCAATGATGGCTGGGGCGCGTACTCGACCGGCACGACAGGCGGGTCAAAAGCATCGTCCTTAAATGTGTATACCGTCAGCAACAGAAACCAGCTTGTCTCGGCATTAGGGAAGGAAACGAACACAACGCCAAAAATCATTTATATCAAGGGAACGATTGACATGAACGTAGATGACAATCTGAAGCCGCTTGGTCTAAATGACTATAAAGATCCGGAGTATGATTTGGACAAATATTTGAAAGCCTATGATCCTAGCACATGGGGCAAAAAAGAGCCGTCGGGAACACAAGAAGAAGCGAGAGCACGCTCTCAGAAAAACCAAAAAGCACGGGTTATGGTGGATATCCCTGCAAACACGACGATCGTCGGTTCAGGGACTAACGCTAAAGTCGTGGGAGGAAACTTCCAAATCAAGAGTGATAACGTCATTATTCGCAACATTGAATTCCAGGATGCCTATGATTATTTTCCGCAATGGGATCCGACTGACGGAAGCTCAGGAAACTGGAACTCACAATACGACAACATCACGATAAACGGCGGCACACACATCTGGATTGATCACTGTACATTTAACGACGGTTCGCGTCCGGACAGCACATCACCGAAATATTATGGAAGAAAATATCAGCACCATGACGGCCAAACGGATGCGTCCAACGGCGCTAACTATATCACGATGTCCTACAACTATTATCACGATCATGATAAAAGCTCCATTTTCGGATCAAGTGACAGCAAAACCTCCGATGACGGCAAATTAAAAATTACGCTCCATCATAACCGCTATAAAAATATTGTCCAGCGCGCGCCGAGAGTCCGCTTCGGGCAAGTGCACGTATACAACAACTATTATGAAGGAAGCACAAGCTCTTCAAGTTATCCTTTCAGCTATGCATGGGGAATCGGAAAGTCATCTAAAATCTATGCCCAAAACAATGTCATTGACGTACCGGGACTGTCAGCTGCTAAAACGATCAGCGTATTCAGCGGGGGAACGGCTTTATATGACTCCGGCACGTTGCTGAACGGCACACAGATCAACGCATCGGCTGCAAACGGGCTGAGCTCTTCTGTCGGCTGGACGCCGTCTCTGCATGGATCGATTGATGCTTCTGCTAATGTGAAATCAAATGTCATAAATCAAGCGGGTGCGGGTAAATTAAATTAA
Amino Acid Sequence of the Secreted Protein Length = 401 aa
MDADLGHQTLGSNDGWGAYSTGTTGGSKASSLNVYTVSNRNQLVSALGKETNTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDPEYDLDKYLKAYDPSTWGKKEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIKSDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSPKYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVIDVPGLSAAKTISVFSGGTALYDSGTLLNGTQINASAANGLSSSVGWTPSLHGSIDASANVKSNVINQAGAGKLN
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide wapA
Signal Peptide Sequence Length = 28 aa
MKKRKRRNFKRFIAAFLVLALMISLVPA
Type Tat
Yield 24.8 (U/ml)
Performance (Current Yield / Highest Yield) 0.08
Charge 8
Charge (N-Region) 8
Hydrophobicity 0.45
Hydrophobicity Plot
Hydrophobicity (H-region) 2.89
Signal Peptide Original Protein Sequence MKKRKRRNFKRFIAAFLVLALMISLVPADVLAKSTEEENGNRIAADDPEETLQKEQTEEAVPFDPKDINKEGEITSERTENTKLYYEGDGVYKQEVYLDPIHTKETPDADWEDISPELKESTSKQVETENAILNSDFQKQMKNGLYATFEHNDHKVTYSLAEAKGPNKTSLTPKDTSADYKTDSNEIVYPDVFPNIDLQTFTFNENIKEDLVLHQYNGYNTFTFQLKTDLQAKEQEDGSIDFSDEKGKVVFSVPKPFMTDSKLDELSGEVERSDKVSYKLEKNEEGYLLHLTADENWLKDPERVYPVSIDPSTSLSVSSDTFVMSAYPTTNYSASSQKWDANLKAYVLKTGYYDKTTGTNYAFMKFNNLKPIQNMTVTKATLKTYVAHSYYGTKATGLWLDTVNSNYDNAKVTWNTKPASKNIGKADVHKGQWASYDVTAAVKSWNSGGANYGFKLHTNGNGKEYWKKLISSANSANKPYIEVTYTIPKGNTPTIKAYHNGDSTGYFDISWKKVEGAKGYKVWIYNGKEYQAISAGNVTSWSTKGKKIWPTSAEIASKRYKLHLDGKDGAELALDPSPVYKNSGGSYATSKNYWIGVSAIFDQGEGAMSAPAKPVIPNVGKAQAPSAKGYNNGNATGYFDLSWKAVSGATGYKVQVFNGKGFETLDLGNQTSWTTKGKKIWPTSAEIKAGKYALHLKDGSGAELPINPGPTYKNAGGDGAKRNYSFKIIAYNKDGEAIASPAATPALPDIARPKNVTGYLYTNTKSSQTGYVNLIWEKVQNAKGYKVNIYNGKEYQSFDVGDADHWTTQNKNIWPTSEEIKAGSYKLHTDGKGGELALDPSPVYNNANGNYKGKKNYSFTLVAYDANGETIPTAPFNPTFHEGAEFLGTEEYWSIIDIPSGQLNGATGNVIVNEEDLSIDGRGPGLGLSRTYNSLDSSDHLFGQGWYADAETSVISTDGGAMYIDEDATTHRFTKKADGTYQPPTGVYLELTETADQFILKTKDQTNAYFNKKGGKLQKVVDGHNNATVYTYNDKNQLTAITDASGRKLTFTYDENGHVTSITGPKNKKVTYSYENDLLKKVTDTDGTVTSYDYDSEGRLVKQYSANSTEAKPVFTEYQYSGHRLEKAINAKKETYVYSYDADKKTLLMTQPNGRKVQYGYNEAGNPIQVIDDAEGLKITTNTKYEGNNVVEDVDPNDVGTGKATESYQYDKDGNVTSVKDAYGTETYEYNKNNDVTKMKDTEGNVTDIAYDGLDAVSETDQSGKSSSAAVYDKYGNQIQSSKDLSASTNILKDGSFEAQKSGWNLTASKDSGKISVIADKSGVLSGSKALEVLSQSTSAGTDHGYSSATQTVELEPNTTYTLSGKIKTDLAKSRAYFNIDLRDKDQKRIQWIHNEYSALAGKNDWTKRQITFTTPANAGKAVVYMEVDHKDKDGKGKAWFDEVQLEKGEVSSSYNPVQNSSFTSATENWNVSGASVDSEEGFNDDVSLKAARTSASQAGSVTKQTVVLGQSANDKPVYLTLTGMSKASSVKFTDEKDYSLQANVTYADGSTGIYNAKFPSGTQEWNRAAVVIPKTKPINKVDISILFQKSATGTVWFDDIRLIEGSLLTKSTYDSNGNYVTKEEDELGYATSTDYDETGKKTSETDAKGEKTTYTYDQADQLTNMTLSNGTSILHSYDKEGNEVSKTIRAGADQTYKFEYDVMGKLVKTTDPLGNVLASEYDANSNLTKTISPNGNEVSLSYDGTDRVKSKSYNGTEKYIFTYDKNGNETSVVNKEQNTTKKRTFDNKNRLTELTDRGGSQTWTYPSDSDKLKTFSWIHGDQKGTNQFTYNKLDQMIEMKDSTSSYSFDYDENGNVQTFITGNGGGTSFSYDERNLVSSLHIGDKNGGDILTESYEYDANGNRTTINSSASGKVQYEYGKLNQLVKETHEDGTVIEYTYDGFGNRKTVTTIKDGSSKTVNASFNIMNQLTKVNDESISYDKNGNRTSDGKFTYTWDAEDNLTAVTKKGEDKPFATYKYDEKGNRIQKTVNGKVTNYFYDGDSLNVLYETDADNNVTKSYTYGDSGQLLSYTENGKKYFYHYNAHGDIIAISDSTGKTVAKYQYDAWGNPTKTEASDEVKDNRYRYAGYQYDEETGLYYLMARYYEPRNGVFLSLDPDPGSDGDSLDQNGYAYGNNNPVMNVDPDGHWVWLVVNAGFAAYDGYKAYKSGKGWKGAAWAAASNFGPGKIFKGASRAYKFTKKAVKITGHTRHGLNQSIGRNGGRGVNLRAKLNAVRSPKKVIKQPNGATKYVGKKATVVLNKRGKVITAYGSSRAKGSKHVFHTHGKGNKSKRRR
Signal Peptide Original DNA Sequence ATGAAAAAAAGAAAGAGGCGAAACTTTAAAAGGTTCATTGCAGCATTTTTAGTGTTGGCTTTAATGATTTCATTAGTGCCAGCCGATGTACTAGCAAAATCTACAGAAGAAGAGAATGGAAACCGTATTGCTGCCGATGATCCAGAAGAAACTCTTCAGAAAGAACAAACTGAGGAAGCTGTACCTTTTGATCCAAAAGATATAAACAAAGAGGGTGAGATCACCTCTGAAAGAACAGAAAATACAAAACTCTATTATGAAGGTGACGGTGTCTATAAACAGGAAGTATACCTTGATCCTATTCATACAAAGGAAACACCGGATGCCGACTGGGAAGACATTTCTCCTGAGTTAAAAGAGTCGACAAGCAAACAAGTCGAGACAGAGAACGCTATTTTGAATTCAGATTTTCAAAAGCAAATGAAGAACGGGCTTTATGCGACTTTTGAGCATAATGACCATAAGGTTACATATTCACTAGCAGAAGCGAAAGGCCCGAATAAAACTTCACTTACTCCGAAAGATACCTCAGCAGATTATAAGACTGACAGTAATGAGATTGTGTATCCGGATGTGTTTCCGAATATTGATCTTCAGACTTTCACATTCAATGAAAACATAAAAGAAGATTTAGTCCTTCATCAGTATAATGGATACAACACATTTACATTTCAGTTAAAAACCGATTTGCAGGCTAAAGAACAAGAGGATGGATCCATTGATTTTTCTGATGAAAAAGGAAAAGTTGTTTTTTCTGTGCCTAAGCCTTTTATGACGGATTCAAAACTAGATGAGCTTTCCGGCGAGGTTGAACGGTCAGACAAAGTCAGCTATAAGCTGGAAAAGAATGAAGAAGGGTATTTGCTTCATCTTACGGCAGACGAGAACTGGCTGAAAGACCCAGAACGCGTATATCCAGTATCTATCGACCCGTCAACTTCTTTATCTGTTTCATCGGATACGTTTGTCATGAGTGCCTACCCGACGACGAACTATTCGGCCTCCTCACAAAAATGGGATGCTAATTTAAAGGCCTATGTACTGAAAACGGGCTATTATGACAAAACAACAGGCACAAACTATGCGTTCATGAAATTTAACAACCTAAAGCCAATTCAAAATATGACTGTCACTAAAGCTACATTAAAAACATACGTAGCCCATTCCTATTATGGAACAAAGGCAACCGGCCTTTGGCTGGATACGGTCAACAGCAATTATGATAATGCAAAAGTGACATGGAACACCAAACCGGCATCTAAAAATATCGGAAAAGCCGACGTCCATAAAGGACAGTGGGCGAGTTATGATGTGACCGCTGCTGTAAAGTCATGGAACTCCGGCGGGGCCAACTATGGATTTAAGCTTCATACAAACGGAAACGGCAAGGAATATTGGAAAAAACTAATTTCTTCAGCGAACTCCGCCAATAAACCATATATTGAAGTGACGTATACCATTCCAAAGGGAAACACACCTACAATCAAAGCCTATCACAACGGCGATTCCACTGGTTATTTCGATATCAGCTGGAAAAAGGTTGAAGGCGCAAAAGGATATAAGGTATGGATTTACAATGGTAAGGAATATCAAGCGATTTCTGCAGGCAACGTCACGTCGTGGAGCACAAAAGGTAAAAAAATCTGGCCTACGAGCGCAGAAATTGCCTCCAAGAGATATAAACTGCATCTGGACGGCAAGGATGGAGCTGAATTGGCATTAGATCCATCCCCGGTCTATAAGAATTCCGGCGGAAGCTATGCTACAAGCAAAAACTATTGGATTGGCGTCAGTGCGATATTTGACCAAGGTGAAGGAGCGATGTCCGCACCGGCAAAGCCCGTTATCCCTAATGTAGGAAAAGCACAAGCACCGTCAGCAAAGGGCTACAATAATGGGAATGCCACAGGATACTTCGACCTTTCTTGGAAAGCTGTATCCGGTGCAACCGGCTATAAGGTTCAGGTGTTCAATGGGAAAGGCTTTGAGACACTTGATCTCGGAAATCAGACGTCTTGGACCACAAAAGGGAAAAAGATCTGGCCGACAAGTGCAGAAATCAAGGCGGGAAAATATGCTCTTCACCTAAAGGACGGAAGCGGAGCAGAGCTGCCGATCAATCCCGGACCAACCTATAAAAACGCCGGGGGAGATGGAGCCAAAAGAAATTACTCGTTTAAAATTATCGCATACAACAAAGACGGCGAAGCCATTGCATCCCCGGCTGCTACCCCGGCACTTCCTGATATCGCTAGGCCAAAGAATGTAACTGGCTATTTGTATACAAATACAAAATCGAGTCAAACGGGTTATGTGAATTTGATATGGGAAAAGGTTCAAAATGCGAAGGGCTATAAAGTTAACATTTACAACGGTAAAGAATATCAGTCCTTTGATGTTGGTGATGCTGATCATTGGACAACCCAAAATAAAAACATCTGGCCGACTTCTGAGGAAATCAAAGCCGGAAGCTATAAGCTTCATACAGATGGAAAAGGCGGGGAATTAGCCCTCGATCCATCACCTGTCTATAACAATGCAAATGGGAATTACAAAGGGAAGAAGAATTATTCTTTCACGCTTGTCGCCTATGATGCGAATGGTGAAACGATTCCAACGGCGCCCTTTAACCCAACGTTCCATGAAGGCGCAGAATTCCTAGGTACAGAAGAGTACTGGTCTATCATTGATATCCCAAGCGGCCAGTTAAATGGTGCGACAGGCAATGTCATTGTGAATGAAGAAGATTTGTCAATTGATGGACGCGGTCCGGGACTTGGCTTATCGAGAACCTACAACAGTCTTGACAGCTCTGATCACTTATTTGGACAAGGCTGGTATGCTGATGCAGAAACATCTGTCATCTCAACGGATGGCGGGGCAATGTATATCGATGAAGACGCTACGACACATCGATTCACGAAAAAAGCCGATGGCACGTATCAGCCGCCGACCGGTGTATATCTTGAATTAACAGAAACAGCTGATCAGTTCATATTAAAAACAAAGGATCAAACCAACGCTTATTTCAATAAAAAAGGCGGCAAACTCCAAAAGGTAGTGGATGGCCATAACAATGCAACTGTCTATACTTACAATGACAAAAATCAACTGACCGCCATTACAGATGCATCAGGCCGCAAGCTTACATTTACCTATGATGAAAACGGCCATGTGACGTCCATCACAGGACCTAAGAATAAGAAAGTCACCTACTCATATGAGAATGATTTGCTGAAGAAGGTTACAGATACAGACGGGACTGTCACAAGCTATGACTACGATAGTGAAGGGCGCCTTGTCAAACAATATTCAGCTAACAGTACCGAAGCAAAGCCTGTCTTTACCGAATATCAGTATAGCGGTCACCGTCTCGAAAAAGCGATCAACGCTAAAAAAGAAACATATGTCTACAGCTATGATGCTGATAAAAAGACGCTTCTCATGACACAGCCAAATGGCCGGAAAGTACAGTATGGCTATAATGAAGCGGGAAATCCGATTCAGGTCATTGATGATGCGGAAGGGTTAAAAATTACAACGAATACAAAGTATGAAGGCAACAATGTTGTAGAAGATGTAGATCCAAATGACGTCGGCACCGGAAAAGCAACGGAAAGCTATCAATATGACAAAGATGGCAACGTTACGTCGGTTAAAGATGCATATGGAACTGAGACATACGAATACAACAAAAACAACGATGTCACAAAGATGAAGGATACCGAGGGCAATGTCACGGATATTGCTTATGACGGACTGGATGCTGTATCCGAAACCGATCAGAGCGGAAAATCATCGTCAGCAGCTGTTTATGATAAATATGGAAACCAGATTCAATCGAGCAAAGATTTATCAGCTTCAACTAATATTTTGAAAGATGGAAGCTTTGAAGCTCAAAAATCCGGCTGGAATCTGACTGCCTCTAAAGACAGCGGCAAAATTTCAGTCATCGCCGATAAAAGCGGAGTTCTTTCCGGATCAAAGGCATTGGAGGTCTTGAGCCAATCAACGTCTGCCGGAACAGATCATGGGTATTCGTCAGCAACACAAACAGTTGAGCTGGAGCCGAATACAACCTATACATTAAGCGGGAAAATCAAAACAGATCTTGCGAAATCAAGAGCCTATTTTAACATTGATTTGCGAGACAAAGATCAAAAACGAATTCAATGGATTCACAATGAATACAGTGCATTAGCCGGAAAAAATGACTGGACAAAACGGCAAATCACTTTTACGACTCCAGCGAATGCAGGCAAAGCTGTTGTGTATATGGAAGTCGATCATAAAGATAAAGATGGCAAAGGAAAAGCATGGTTTGATGAAGTCCAGCTGGAAAAAGGCGAAGTATCATCAAGCTATAACCCAGTTCAAAACAGCAGCTTCACATCAGCAACGGAAAACTGGAATGTAAGCGGAGCATCCGTTGATTCTGAAGAAGGCTTTAATGATGATGTATCATTAAAAGCTGCCCGGACCAGTGCGTCCCAAGCCGGATCAGTAACAAAACAAACCGTTGTCTTAGGGCAAAGCGCAAATGACAAACCGGTTTATCTCACACTGACAGGAATGTCGAAAGCAAGCAGCGTCAAATTTACAGATGAAAAAGACTATTCTCTGCAGGCGAATGTCACTTACGCTGACGGCAGCACAGGCATCTACAATGCGAAATTCCCTTCTGGAACACAGGAATGGAACCGAGCAGCTGTCGTCATCCCTAAAACAAAGCCGATCAATAAAGTCGATATCAGTATTCTGTTCCAAAAGAGTGCGACAGGCACAGTCTGGTTTGATGATATCCGTTTGATTGAAGGAAGCCTTCTGACTAAAAGCACGTATGACAGTAATGGCAACTATGTGACCAAGGAAGAGGACGAACTAGGTTACGCCACTTCAACTGATTACGATGAAACTGGTAAGAAAACGTCTGAAACAGATGCAAAAGGTGAAAAAACAACCTATACGTATGATCAGGCAGACCAATTGACAAACATGACGCTCTCTAACGGCACGTCTATCCTTCACAGCTATGATAAGGAAGGCAATGAGGTAAGCAAAACCATCCGGGCAGGAGCAGATCAAACCTATAAATTCGAATATGATGTCATGGGCAAGCTTGTCAAAACAACTGACCCTCTCGGCAATGTGTTAGCAAGCGAATATGATGCCAACAGCAACCTGACCAAAACCATTTCACCAAATGGCAACGAAGTGTCTCTTTCTTATGATGGAACAGACCGGGTGAAATCAAAGTCGTATAACGGTACAGAAAAGTACATTTTCACGTATGACAAAAATGGAAATGAAACGTCGGTCGTCAATAAAGAGCAGAACACCACAAAGAAACGGACATTTGACAACAAAAACCGTTTAACCGAACTGACAGACCGCGGCGGCAGCCAAACTTGGACATATCCTAGTGACTCCGATAAGCTGAAAACCTTCTCGTGGATTCATGGGGACCAAAAAGGAACGAATCAATTTACGTATAACAAGCTGGATCAAATGATTGAGATGAAAGACAGTACATCCTCATATTCATTTGATTATGATGAAAATGGAAACGTCCAAACCTTTATTACGGGCAACGGCGGCGGAACAAGTTTTTCATATGACGAAAGAAACCTTGTAAGCTCGCTGCATATCGGTGACAAAAACGGCGGAGACATTCTGACGGAAAGCTATGAATATGATGCCAACGGCAACCGCACAACGATAAACAGCTCAGCAAGCGGCAAGGTGCAATATGAGTATGGGAAGCTGAATCAGCTTGTCAAAGAAACCCATGAAGACGGTACTGTTATTGAATACACATATGATGGATTCGGAAACCGGAAAACCGTTACAACTATAAAAGACGGCTCAAGCAAAACGGTGAACGCTTCCTTCAACATCATGAACCAGCTGACAAAAGTCAATGATGAAAGCATCTCTTACGATAAAAACGGCAACCGCACATCCGACGGCAAATTCACATATACGTGGGATGCAGAAGATAACCTGACCGCCGTTACCAAAAAAGGCGAAGACAAGCCATTCGCAACATATAAGTATGACGAAAAAGGAAACAGAATCCAAAAAACCGTCAACGGAAAAGTCACGAACTACTTCTACGACGGAGACAGCTTGAACGTCTTGTATGAAACAGACGCGGACAACAACGTCACAAAATCATACACGTACGGAGACAGCGGCCAGCTGTTATCCTACACAGAAAATGGTAAGAAATATTTCTATCACTACAACGCTCACGGCGACATCATCGCGATCTCAGACAGCACTGGAAAAACCGTTGCCAAGTATCAATACGACGCATGGGGAAATCCAACAAAAACCGAAGCAAGCGACGAAGTAAAAGACAATCGCTACCGCTACGCAGGCTACCAATACGACGAAGAAACCGGCCTGTACTATCTCATGGCCCGCTACTACGAGCCAAGAAACGGCGTGTTCCTATCGCTCGACCCAGACCCGGGCAGCGACGGAGATTCATTGGATCAGAATGGGTATGCTTATGGAAATAATAACCCAGTGATGAATGTTGATCCGGACGGGCATTGGGTATGGCTTGTGGTAAATGCCGGATTTGCAGCCTATGATGGATATAAGGCATATAAATCAGGTAAAGGATGGAAAGGTGCTGCTTGGGCAGCTGCTTCTAATTTTGGTCCTGGGAAGATATTTAAGGGTGCTAGTCGCGCCTATAAGTTTACGAAGAAGGCAGTTAAAATCACAGGTCATACAAGACATGGACTTAATCAATCTATAGGAAGAAATGGAGGCCGTGGAGTCAATTTAAGGGCCAAACTGAATGCTGTGCGAAGCCCTAAAAAAGTAATAAAACAACCTAATGGGGCAACGAAATATGTTGGAAAAAAAGCTACAGTTGTTTTAAATAAAAGGGGTAAAGTAATCACAGCTTATGGAAGTTCAAGAGCTAAGGGTTCTAAACACGTATTCCATACACACGGAAAAGGAAATAAATCTAAGAGAAGGCGTTAA
Reference Zhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 [PubMed]
Miscellaneous -