SPSED Signal Peptide Secretion Efficiency Database

Detail Information for SPSED0009003

Accession Number SPSED0009003
Secreted Protein Alkaline polygalacturonate lyase (PGL)
Uniprot Accession Number -
GenBank Accession Number -
Source Organism of the Secreted Protein Bacillus subtilis WSHB04-02
Nucleotide Sequence of the Secreted Protein Length = 1206 nt
ATGGATGCTGATTTAGGCCACCAGACGTTGGGATCCAATGATGGCTGGGGCGCGTACTCGACCGGCACGACAGGCGGGTCAAAAGCATCGTCCTTAAATGTGTATACCGTCAGCAACAGAAACCAGCTTGTCTCGGCATTAGGGAAGGAAACGAACACAACGCCAAAAATCATTTATATCAAGGGAACGATTGACATGAACGTAGATGACAATCTGAAGCCGCTTGGTCTAAATGACTATAAAGATCCGGAGTATGATTTGGACAAATATTTGAAAGCCTATGATCCTAGCACATGGGGCAAAAAAGAGCCGTCGGGAACACAAGAAGAAGCGAGAGCACGCTCTCAGAAAAACCAAAAAGCACGGGTTATGGTGGATATCCCTGCAAACACGACGATCGTCGGTTCAGGGACTAACGCTAAAGTCGTGGGAGGAAACTTCCAAATCAAGAGTGATAACGTCATTATTCGCAACATTGAATTCCAGGATGCCTATGATTATTTTCCGCAATGGGATCCGACTGACGGAAGCTCAGGAAACTGGAACTCACAATACGACAACATCACGATAAACGGCGGCACACACATCTGGATTGATCACTGTACATTTAACGACGGTTCGCGTCCGGACAGCACATCACCGAAATATTATGGAAGAAAATATCAGCACCATGACGGCCAAACGGATGCGTCCAACGGCGCTAACTATATCACGATGTCCTACAACTATTATCACGATCATGATAAAAGCTCCATTTTCGGATCAAGTGACAGCAAAACCTCCGATGACGGCAAATTAAAAATTACGCTCCATCATAACCGCTATAAAAATATTGTCCAGCGCGCGCCGAGAGTCCGCTTCGGGCAAGTGCACGTATACAACAACTATTATGAAGGAAGCACAAGCTCTTCAAGTTATCCTTTCAGCTATGCATGGGGAATCGGAAAGTCATCTAAAATCTATGCCCAAAACAATGTCATTGACGTACCGGGACTGTCAGCTGCTAAAACGATCAGCGTATTCAGCGGGGGAACGGCTTTATATGACTCCGGCACGTTGCTGAACGGCACACAGATCAACGCATCGGCTGCAAACGGGCTGAGCTCTTCTGTCGGCTGGACGCCGTCTCTGCATGGATCGATTGATGCTTCTGCTAATGTGAAATCAAATGTCATAAATCAAGCGGGTGCGGGTAAATTAAATTAA
Amino Acid Sequence of the Secreted Protein Length = 401 aa
MDADLGHQTLGSNDGWGAYSTGTTGGSKASSLNVYTVSNRNQLVSALGKETNTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDPEYDLDKYLKAYDPSTWGKKEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIKSDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSPKYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVIDVPGLSAAKTISVFSGGTALYDSGTLLNGTQINASAANGLSSSVGWTPSLHGSIDASANVKSNVINQAGAGKLN
Expression Host Bacillus subtilis WB600

Source Organism of the Signal Peptide Bacillus subtilis
Source Gene of the Signal Peptide nprE
Signal Peptide Sequence Length = 27 aa
MGLGKKLSVAVAASFMSLSISLPGVQA
Type Sec
Yield 23.3 (U/ml)
Performance (Current Yield / Highest Yield) 0.08
Charge 2
Charge (N-Region) 2
Hydrophobicity 1.04
Hydrophobicity Plot
Hydrophobicity (H-region) 1.58
Signal Peptide Original Protein Sequence MGLGKKLSVAVAASFMSLSISLPGVQAAEGHQLKENQTNFLSKNAIAQSELSAPNDKAVKQFLKKNSNIFKGDPSKRLKLVESTTDALGYKHFRYAPVVNGVPIKDSQVIVHVDKSDNVYAVNGELHNQSAAKTDNSQKVSSEKALALAFKAIGKSPDAVSNGAAKNSNKAELKAIETKDGSYRLAYDVTIRYVEPEPANWEVLVDAETGSILKQQNKVEHAAATGSGTTLKGATVPLNISYEGGKYVLRDLSKPTGTQIITYDLQNRQSRLPGTLVSSTTKTFTSSSQRAAVDAHYNLGKVYDYFYSNFKRNSYDNKGSKIVSSVHYGTQYNNAAWTGDQMIYGDGDGSFFSPLSGSLDVTAHEMTHGVTQETANLIYENQPGALNESFSDVFGYFNDTEDWDIGEDITVSQPALRSLSNPTKYNQPDNYANYRNLPNTDEGDYGGVHTNSGIPNKAAYNTITKLGVSKSQQIYYRALTTYLTPSSTFKDAKAALIQSARDLYGSTDAAKVEAAWNAVGL
Signal Peptide Original DNA Sequence GTGGGTTTAGGTAAGAAATTGTCTGTTGCTGTCGCTGCTTCGTTTATGAGTTTATCAATCAGCCTGCCAGGTGTTCAGGCTGCTGAAGGTCATCAGCTTAAAGAGAATCAAACAAATTTCCTCTCCAAAAACGCGATTGCGCAATCAGAACTCTCTGCACCAAATGACAAGGCTGTCAAGCAGTTTTTGAAAAAGAACAGCAACATTTTTAAAGGTGACCCTTCCAAAAGGCTGAAGCTTGTTGAAAGCACGACTGATGCCCTTGGATACAAGCACTTTCGATATGCGCCTGTCGTTAACGGAGTGCCAATTAAAGATTCGCAAGTGATCGTTCACGTCGATAAATCCGATAATGTCTATGCGGTCAATGGTGAATTACACAATCAATCTGCTGCAAAAACAGATAACAGCCAAAAAGTCTCTTCTGAAAAAGCGCTGGCACTCGCTTTCAAAGCTATCGGCAAATCACCAGACGCTGTTTCTAACGGAGCGGCCAAAAACAGCAATAAAGCCGAATTAAAAGCGATAGAAACAAAAGACGGCAGCTATCGTCTTGCTTACGACGTGACGATTCGCTATGTCGAGCCTGAACCTGCAAACTGGGAAGTCTTAGTTGACGCCGAAACAGGCAGCATTTTAAAACAGCAAAATAAAGTAGAACATGCCGCCGCCACTGGAAGCGGAACAACGCTAAAGGGCGCAACTGTTCCTTTGAACATCTCTTATGAAGGCGGAAAATATGTTCTAAGAGATCTTTCAAAACCAACAGGCACCCAAATCATCACATATGATTTGCAAAACAGACAAAGCCGCCTTCCGGGCACGCTTGTCTCAAGCACAACGAAAACATTTACATCTTCATCACAGCGGGCAGCCGTTGACGCACACTATAACCTCGGTAAAGTGTACGATTATTTTTATTCAAACTTTAAACGAAACAGCTATGATAACAAAGGCAGTAAAATCGTTTCTTCCGTTCACTACGGCACTCAATACAATAACGCTGCATGGACAGGAGACCAGATGATTTACGGTGATGGCGACGGTTCATTCTTCTCTCCGCTTTCCGGCTCATTAGATGTGACAGCGCATGAAATGACACATGGCGTCACCCAAGAAACAGCCAACTTGATTTATGAAAATCAGCCAGGTGCATTAAACGAGTCTTTCTCTGACGTATTCGGGTATTTTAACGATACAGAAGACTGGGACATCGGTGAAGACATTACGGTCAGCCAGCCTGCTCTTCGCAGCCTGTCCAACCCTACAAAATACAACCAGCCTGACAATTACGCCAATTACCGAAACCTTCCAAACACAGATGAAGGCGATTATGGCGGTGTACACACAAACAGCGGAATTCCAAACAAAGCCGCTTACAACACCATCACAAAACTTGGTGTATCTAAATCACAGCAAATCTATTACCGTGCGTTAACAACGTACCTCACGCCTTCTTCCACGTTCAAAGATGCCAAGGCAGCTCTCATTCAGTCTGCCCGTGACCTCTACGGCTCAACTGATGCCGCTAAAGTTGAAGCAGCCTGGAATGCTGTTGGATTGTAA
Reference Zhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 [PubMed]
Miscellaneous -