SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0009006
| Accession Number | SPSED0009006 |
| Secreted Protein | Alkaline polygalacturonate lyase (PGL) |
| Uniprot Accession Number | - |
| GenBank Accession Number | - |
| Source Organism of the Secreted Protein | Bacillus subtilis WSHB04-02 | Nucleotide Sequence of the Secreted Protein | Length = 1206 ntATGGATGCTGATTTAGGCCACCAGACGTTGGGATCCAATGATGGCTGGGGCGCGTACTCGACCGGCACGACAGGCGGGTCAAAAGCATCGTCCTTAAATGTGTATACCGTCAGCAACAGAAACCAGCTTGTCTCGGCATTAGGGAAGGAAACGAACACAACGCCAAAAATCATTTATATCAAGGGAACGATTGACATGAACGTAGATGACAATCTGAAGCCGCTTGGTCTAAATGACTATAAAGATCCGGAGTATGATTTGGACAAATATTTGAAAGCCTATGATCCTAGCACATGGGGCAAAAAAGAGCCGTCGGGAACACAAGAAGAAGCGAGAGCACGCTCTCAGAAAAACCAAAAAGCACGGGTTATGGTGGATATCCCTGCAAACACGACGATCGTCGGTTCAGGGACTAACGCTAAAGTCGTGGGAGGAAACTTCCAAATCAAGAGTGATAACGTCATTATTCGCAACATTGAATTCCAGGATGCCTATGATTATTTTCCGCAATGGGATCCGACTGACGGAAGCTCAGGAAACTGGAACTCACAATACGACAACATCACGATAAACGGCGGCACACACATCTGGATTGATCACTGTACATTTAACGACGGTTCGCGTCCGGACAGCACATCACCGAAATATTATGGAAGAAAATATCAGCACCATGACGGCCAAACGGATGCGTCCAACGGCGCTAACTATATCACGATGTCCTACAACTATTATCACGATCATGATAAAAGCTCCATTTTCGGATCAAGTGACAGCAAAACCTCCGATGACGGCAAATTAAAAATTACGCTCCATCATAACCGCTATAAAAATATTGTCCAGCGCGCGCCGAGAGTCCGCTTCGGGCAAGTGCACGTATACAACAACTATTATGAAGGAAGCACAAGCTCTTCAAGTTATCCTTTCAGCTATGCATGGGGAATCGGAAAGTCATCTAAAATCTATGCCCAAAACAATGTCATTGACGTACCGGGACTGTCAGCTGCTAAAACGATCAGCGTATTCAGCGGGGGAACGGCTTTATATGACTCCGGCACGTTGCTGAACGGCACACAGATCAACGCATCGGCTGCAAACGGGCTGAGCTCTTCTGTCGGCTGGACGCCGTCTCTGCATGGATCGATTGATGCTTCTGCTAATGTGAAATCAAATGTCATAAATCAAGCGGGTGCGGGTAAATTAAATTAA |
| Amino Acid Sequence of the Secreted Protein | Length = 401 aaMDADLGHQTLGSNDGWGAYSTGTTGGSKASSLNVYTVSNRNQLVSALGKETNTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDPEYDLDKYLKAYDPSTWGKKEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIKSDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSPKYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTSDDGKLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSSYPFSYAWGIGKSSKIYAQNNVIDVPGLSAAKTISVFSGGTALYDSGTLLNGTQINASAANGLSSSVGWTPSLHGSIDASANVKSNVINQAGAGKLN |
| Expression Host | Bacillus subtilis WB600 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | amyX |
| Signal Peptide Sequence | Length = 30 aaMVSIRRSFEAYVDDMNIITVLIPAEQKEIM |
| Type | Tat |
| Yield | 16 (U/ml) |
| Performance (Current Yield / Highest Yield) | 0.05 |
| Charge | -2 |
| Charge (N-Region) | -1 |
| Hydrophobicity | 0.28 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 1.96 |
| Signal Peptide Original Protein Sequence | MVSIRRSFEAYVDDMNIITVLIPAEQKEIMTPPFRLETEITDFPLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEKGVYAVTVTGDLHGYEYLFCICNNSEWMETVDQYAKAVTVNGEKGVVLRPDQMKWTAPLKPFSHPVDAVIYETHLRDFSIHENSGMINKGKYLALTETDTQTANGSSSGLAYVKELGVTHVELLPVNDFAGVDEEKPLDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKRENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDLLGILDIDTVLYMKEKATKAKPGILLFGEGWDLATPLPHEQKAALANAPRMPGIGFFNDMFRDAVKGNTFHLKATGFALGNGESAQAVMHGIAGSSGWKALAPIVPEPSQSINYVESHDNHTFWDKMSFALPQENDSRKRSRQRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLDWDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDLDEVDEWKDIIVIHHASPDSVEWRLPNDIPYRLLCDPSGFQEDPTEIKKTVAVNGIGTVILYLASDLKSFA |
| Signal Peptide Original DNA Sequence | ATGGTCAGCATCCGCCGCAGCTTCGAAGCGTATGTCGATGACATGAATATCATTACTGTTCTGATTCCTGCTGAACAAAAGGAAATCATGACACCGCCGTTTCGGCTTGAGACAGAAATAACAGATTTTCCTCTGGCTGTCAGGGAGGAATACTCCCTTGAAGCAAAATACAAGTACGTCTGCGTATCCGACCATCCTGTGACATTTGGAAAAATCCATTGCGTCAGAGCATCCAGCGGCCACAAAACGGATCTCCAAATTGGCGCGGTCATCCGGACGGCAGCGTTTGATGACGAATTTTATTATGACGGAGAGCTGGGCGCCGTTTATACCGCGGATCATACCGTATTTAAAGTATGGGCGCCTGCTGCAACCTCAGCTGCTGTCAAGCTTTCACACCCCAATAAAAGCGGGCGCACATTCCAAATGACTCGCTTGGAAAAAGGCGTCTATGCCGTTACGGTCACAGGTGACCTTCACGGATATGAGTATTTGTTTTGCATCTGCAACAATTCAGAATGGATGGAAACAGTTGACCAGTATGCCAAGGCTGTGACTGTAAATGGAGAGAAGGGCGTCGTCTTGCGCCCGGATCAAATGAAATGGACTGCTCCTCTTAAACCATTCTCACACCCTGTGGATGCCGTCATCTATGAGACGCATCTTCGCGACTTCTCCATCCATGAAAACAGCGGCATGATAAACAAGGGAAAATACTTAGCGCTGACGGAAACTGATACACAAACCGCAAATGGCAGTTCTTCGGGATTAGCGTATGTAAAAGAGCTTGGTGTGACACATGTGGAGCTTCTGCCGGTGAATGATTTTGCCGGAGTTGATGAAGAGAAGCCGCTTGATGCATACAATTGGGGATATAACCCGCTTCATTTCTTTGCCCCGGAGGGAAGCTATGCCTCAAATCCTCATGATCCTCAAACGAGAAAAACAGAGCTGAAACAAATGATCAATACCCTGCATCAGCACGGTCTGCGAGTCATTCTGGATGTTGTTTTTAACCATGTGTATAAGAGGGAGAATTCCCCCTTTGAAAAGACAGTGCCCGGTTATTTTTTCCGGCACGACGAATGTGGGATGCCATCAAACGGCACCGGCGTTGGCAATGATATTGCATCAGAAAGAAGGATGGCAAGAAAATTCATTGCGGATTGCGTGGTCTATTGGCTTGAAGAATACAATGTTGACGGCTTCCGCTTTGATCTCCTCGGGATTTTAGATATTGACACCGTGCTTTATATGAAAGAGAAAGCAACTAAGGCAAAGCCCGGAATCCTGCTTTTTGGAGAAGGGTGGGACCTGGCTACACCGCTGCCGCATGAACAGAAAGCTGCTTTGGCGAACGCGCCAAGAATGCCGGGCATCGGCTTTTTTAATGATATGTTTCGTGACGCTGTAAAAGGGAACACCTTTCACCTTAAGGCAACAGGGTTTGCGCTCGGCAACGGTGAATCAGCACAAGCTGTGATGCATGGAATTGCCGGGTCTTCCGGATGGAAGGCATTAGCACCGATTGTTCCGGAACCAAGCCAGTCCATCAATTATGTCGAATCACACGACAATCACACCTTTTGGGATAAAATGAGCTTTGCGCTTCCTCAAGAAAATGACAGCCGAAAGCGAAGCAGGCAAAGGCTTGCAGCCGCGATTATTTTGCTTGCCCAAGGGGTGCCGTTTATTCACAGCGGCCAGGAATTTTTCCGGACGAAGCAGGGAGTGGAAAACAGCTATCAATCCAGTGACAGCATCAACCAGCTCGACTGGGATCGCCGTGAAACATTCAAAGAAGATGTTCACTATATCCGCAGGCTGATCTCGCTGAGAAAAGCGCATCCTGCATTCCGTCTTAGGTCCGCTGCAGACATCCAGCGCCATCTTGAATGCTTGACGCTAAAAGAACACCTTATCGCATACAGGCTTTATGATCTTGACGAGGTTGACGAATGGAAAGATATCATTGTTATCCATCACGCGAGTCCAGACTCCGTCGAGTGGAGGCTGCCAAACGACATACCTTATCGGCTTTTATGTGATCCATCAGGATTTCAGGAAGACCCAACAGAAATCAAGAAAACGGTTGCAGTAAACGGCATCGGAACGGTTATCTTATATTTAGCATCAGATCTTAAGAGTTTTGCTTGA |
| Reference | Zhang J J, Kang Z, Ling Z M, et al. High-level extracellular production of alkaline polygalacturonate lyase in Bacillus subtilis with optimized regulatory elements[J]. Bioresource Technology, 2013, 146: 543-548 [PubMed] |
| Miscellaneous | - |