SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0012027
| Accession Number | SPSED0012027 |
| Secreted Protein | Cutinase |
| Uniprot Accession Number | P00590 |
| GenBank Accession Number | AAA33335.1 |
| Source Organism of the Secreted Protein | Fusarium solani pisi |
| Nucleotide Sequence of the Secreted Protein | Length = 693 nt ATGAAATTCTTCGCTCTCACCACACTTCTCGCCGCCACGGCTTCGGCTCTGCCTACTTCTAACCCTGCCCAGGAGCTTGAGGCGCGCCAGCTTGGTAGAACAACTCGCGACGATCTGATCAACGGCAATAGCGCTTCCTGCGCCGATGTCATCTTCATTTATGCCCGAGGTTCAACAGAGACGGGCAACTTGGGAACTCTCGGTCCTAGCATTGCCTCCAACCTTGAGTCCGCCTTCGGCAAGGACGGTGTCTGGATTCAGGGCGTTGGCGGTGCCTACGCAGCCACTCTTGGAGACAATGCTCTCCCTCGCGGAACCTCTAGCGCCGCAATCAGGGAGATGCTCGGTCTCTTCCAGCAGGCCAACACCAAGTGCCCTGACGCGACTTTGATCGCCGGTGGCTACAGCCAGGGTGCTGCACTTGCAGCCGCCTCCATCGAGGACCTCGACTCGGCCATTCGTGACAAGATCGCCGGAACTGTTCTGTTCGGCTACACCAAGAACCTACAGAACCGTGGCCGAATCCCCAACTACCCTGCCGACAGGACCAAGGTCTTCTGCAATACAGGGGATCTCGTTTGTACTGGTAGCTTGATCGTTGCTGCACCTCACTTGGCTTATGGTCCTGATGCTCGTGGCCCTGCCCCTGAGTTCCTCATCGAGAAGGTTCGGGCTGTCCGTGGTTCTGCTTGA |
| Amino Acid Sequence of the Secreted Protein | Length = 230 aa MKFFALTTLLAATASALPTSNPAQELEARQLGRTTRDDLINGNSASCRDVIFIYARGSTETGNLGTLGPSIASNLESAFGKDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNTGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA |
| Expression Host | Corynebacterium glutamicum |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | yobV |
| Signal Peptide Sequence | Length = 21 aa MKLERLLAMVVLLISKKQVQA |
| Type | Sec |
| Yield | 5 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.38 |
| Charge | 3 |
| Charge (N-Region) | 1 |
| Hydrophobicity | 0.76 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 3.53 |
| Signal Peptide Original Protein Sequence | MKLERLLAMVVLLISKKQVQAAELAELFEVSVRTIYRDIETINRAGIPIVTSQGSGGGIGIMETYRLEREWLKEEELFAIASALQSVSSMYEPVSHSTAYQKIKHLIPEQSTQAFKHQTEKWFIDMTAWGHTEDQKTLREKISAAIDRLLTISFTYTSASGETLLRETEPYTLVCKAGHWYLYAYCLVRNDFRFFKLNRMKDLAILHQTFIRKDIQLDTLPWDKSWYQKDRLTELVILVQPSARQRIGEWFGYDVLHCCEEDEICQAVISLPEDQWLIGFLLQFGKDIEVLQPLHIRDKVKETIHHMQKIYET |
| Signal Peptide Original DNA Sequence | ATGAAACTGGAACGTTTGTTAGCGATGGTTGTGTTATTGATCAGCAAAAAACAAGTGCAGGCTGCAGAGCTTGCTGAGCTGTTTGAAGTATCTGTCAGAACAATTTACCGTGATATTGAGACGATCAATAGGGCTGGTATTCCGATTGTGACCTCACAGGGTTCCGGGGGCGGAATTGGCATCATGGAAACGTATCGGCTTGAAAGAGAGTGGCTGAAGGAGGAGGAATTGTTTGCAATTGCTTCCGCTTTGCAAAGCGTTTCATCTATGTATGAACCCGTTTCTCACAGCACAGCCTATCAAAAAATCAAACACCTTATACCTGAGCAATCTACTCAAGCATTTAAGCACCAAACAGAAAAATGGTTTATTGACATGACGGCTTGGGGCCATACTGAGGATCAAAAGACGCTGCGCGAGAAAATCAGCGCTGCCATCGATCGGTTGTTAACGATATCTTTTACATATACAAGCGCAAGCGGGGAAACACTACTTCGGGAAACTGAACCTTATACATTGGTTTGCAAGGCGGGGCACTGGTATTTATATGCCTACTGCCTGGTAAGAAATGATTTTCGCTTTTTTAAACTGAACCGGATGAAAGATCTGGCCATCTTACATCAGACATTTATCAGAAAAGACATTCAGCTGGACACTTTGCCATGGGATAAGAGTTGGTACCAAAAGGACCGTTTAACTGAATTGGTTATTCTTGTTCAGCCGTCAGCACGTCAGCGTATAGGTGAATGGTTCGGATATGATGTCCTTCACTGTTGTGAGGAAGATGAAATCTGCCAGGCGGTGATTTCACTTCCAGAAGATCAATGGCTGATCGGTTTTTTGCTCCAGTTCGGAAAGGATATTGAGGTTCTGCAGCCTCTTCATATAAGGGATAAAGTAAAAGAAACCATTCATCACATGCAAAAAATATATGAAACCTGA |
| Reference | Hemmerich J, Rohe P, Kleine B, et al. Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum[J]. Microbial Cell Factories, 2016, 15(1): 208. [PubMed] |
| Miscellaneous | - |