SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0013100
| Accession Number | SPSED0013100 |
| Secreted Protein | Alkaline active xylanase (XynBYG) (promoter：Pglvm) |
| Uniprot Accession Number | A0A172WBP7 |
| GenBank Accession Number | ANF06879.1 |
| Source Organism of the Secreted Protein | Bacillus pumilus BYG |
| Nucleotide Sequence of the Secreted Protein | Length = 687 nt ATGAATTTGAGAAAATTAAGACTGTTGTTTGTGATGTGTATTGGACTGACGCTTATACTGACGGCTGTACCAGCCCATGCGAGAACCATTACGAATAATGAAATGGGTAACCATAGCGGGTACGATTATGAATTATGGAAGGATTATGGAAATACCTCGATGACACTCAATAACGGCGGGGCATTTAGTGCAGGCTGGAACAATATCGGAAATGCTTTATTTAGAAAAGGGAAAAAGTTTGATTCCACTAGAACTCACCATCAGCTTGGCAACATCTCCATCAATTACAACGCAAGTTTTAACCCAGGCGGGAATTCCTATCTATGTGTCTATGGCTGGACACAATCTCCATTAGCAGAATACTACATTGTTGATTCATGGGGCACGTATCGTCCAACAGGAGCGTATAAAGGATCATTTTATGCTGATGGAGGCACATATGACATTTATGAAACAACCCGTGTCAATCAGCCTTCCATTATCGGGATCGCAACCTTCAAGCAATATTGGAGTGTACGTCAAACGAAACGTACAAGCGGAACGGTCTCCGTCAGTGCGCATTTTAGAAAATGGGAAAGCTTAGGGATGCCAATAGGGAAAATGTATGAAACGGCATTTACTGTAGAAGGCTACCAAAGCAGCGGAAGTGCAAATGTGATGACCAATCAGCTGTTTATTGGCAACTAA |
| Amino Acid Sequence of the Secreted Protein | Length = 228 aa MNLRKLRLLFVMCIGLTLILTAVPAHARTITNNEMGNHSGYDYELWKDYGNTSMTLNNGGAFSAGWNNIGNALFRKGKKFDSTRTHHQLGNISINYNASFNPGGNSYLCVYGWTQSPLAEYYIVDSWGTYRPTGAYKGSFYADGGTYDIYETTRVNQPSIIGIATFKQYWSVRQTKRTSGTVSVSAHFRKWESLGMPIGKMYETAFTVEGYQSSGSANVMTNQLFIGN |
| Expression Host | Bacillus subtilis WB700 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | motB |
| Signal Peptide Sequence | Length = 40 aa MARKKKKKHEDEHVDESWLVPYADILTLLLALFIVLYASS |
| Type | Sec |
| Yield | 13.8 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.04 |
| Charge | 0 |
| Charge (N-Region) | 1 |
| Hydrophobicity | 0.01 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 0.94 |
| Signal Peptide Original Protein Sequence | MARKKKKKHEDEHVDESWLVPYADILTLLLALFIVLYASSSIDAAKFQMLSKSFNEVFTGGTGVLDYSSVTPPENESDGIDEVKKEKEEKEKNKKEKEKAADQEELENVKSQVEKFIKDKKLEHQLETKMTSEGLLITIKDSIFFDSGKATIRKEDVPLAKEISNLLVINPPRNIIISGHTDNMPIKNSEFQSNWHLSVMRAVNFMGLLIENPKLDAKVFSAKGYGEYKPVASNKTAEGRSKNRRVEVLILPRGAAETNEK |
| Signal Peptide Original DNA Sequence | ATGGCGAGAAAAAAGAAGAAGAAGCATGAGGACGAGCACGTTGATGAATCATGGCTCGTTCCTTACGCCGACATCCTTACTCTTCTCCTGGCATTGTTTATTGTGCTGTACGCGAGCAGCTCGATTGACGCAGCTAAGTTTCAAATGCTCTCAAAATCATTTAATGAAGTTTTTACAGGCGGAACCGGTGTACTGGACTACTCCAGTGTCACTCCTCCTGAAAACGAGTCAGACGGCATCGATGAAGTGAAAAAGGAAAAAGAAGAGAAAGAGAAAAACAAGAAAGAAAAAGAAAAAGCAGCTGATCAAGAAGAACTTGAAAATGTGAAGAGCCAGGTGGAAAAGTTCATCAAAGATAAAAAGCTGGAACATCAGCTGGAGACGAAGATGACTAGTGAAGGCCTTCTGATTACGATTAAAGACAGCATCTTCTTCGATTCTGGAAAAGCGACCATCCGTAAGGAAGATGTGCCGCTTGCAAAAGAGATTTCAAATCTTCTTGTGATTAACCCGCCAAGAAATATCATTATCAGCGGACATACTGATAATATGCCAATTAAAAATTCTGAATTCCAATCAAACTGGCATTTAAGCGTCATGAGAGCGGTAAACTTTATGGGGCTCCTGATTGAAAACCCCAAGCTCGATGCAAAAGTGTTCAGCGCCAAGGGTTATGGCGAGTATAAACCGGTGGCTTCCAATAAAACTGCGGAAGGCCGAAGCAAAAACCGGCGGGTTGAAGTTCTCATTTTGCCGAGAGGCGCAGCGGAAACAAATGAAAAATAG |
| Reference | Zhang W, Yang M, Yang Y, et al. Optimal secretion of alkali-tolerant xylanase in Bacillus subtilis by signal peptide screening[J]. Applied Microbiology and Biotechnology, 2016, 100(20): 8745-8756 [PubMed] |
| Miscellaneous | promoter：Pglvm |