SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0016023
| Accession Number | SPSED0016023 |
| Secreted Protein | Alkaline protease (alk) |
| Uniprot Accession Number | P41362 |
| GenBank Accession Number | AAC60420.1 |
| Source Organism of the Secreted Protein | Bacillus alcalophilus |
| Nucleotide Sequence of the Secreted Protein | Length = 1143 nt ATGAAGAAACCGTTGGGGAAAATTGTCGCAAGCACCGCACTACTCATTTCTGTTGCTTTTAGTTCATCGATCGCATCGGCTGCTGAAGAAGCAAAAGAAAAATATTTAATTGGCTTTAATGAGCAGGAAGCTGTTAGTGAGTTTGTAGAACAAGTAGAGGCAAATGACGAGGTCGCCATTCTCTCTGAGGAAGAGGAAGTCGAAATTGAATTGCTTCATGAATTTGAAACGATTCCTGTTTTATCCGTTGAGTTAAGCCCAGAAGATGTGGACGCGCTTGAACTCGATCCAGCGATTTCTTATATTGAAGAGGATGCAGAAGTAACGACAATGGCGCAATCAGTGCCATGGGGAATTAGCCGTGTGCAAGCCCCAGCTGCCCATAACCGTGGATTGACAGGTTCTGGTGTAAAAGTTGCTGTCCTCGATACAGGTATTTCCACTCATCCAGACTTAAATATTCGTGGTGGCGCTAGCTTTGTACCAGGGGAACCATCCACTCAAGATGGGAATGGGCATGGCACGCATGTGGCCGGGACGATTGCTGCTTTAAACAATTCGATTGGCGTTCTTGGCGTAGCGCCGAGCGCGGAACTATACGCTGTTAAAGTATTAGGGGCGAGCGGTTCAGGTTCGGTCAGCTCGATTGCCCAAGGATTGGAATGGGCAGGGAACAATGGCATGCACGTTGCTAATTTGAGTTTAGGAAGCCCTTCGCCAAGTGCCACACTTGAGCAAGCTGTTAATAGCGCGACTTCTAGAGGGGTTCTTGTTGTAGCGGCATCTGGGAATTCAGGTGCAGGCTCAATCAGCTATCCGGCCCGTTATGCGAACGCAATGGCAGTCGGAGCGACTGACCAAAACAACAACCGCGCCAGCTTTTCACAGTATGGCGCAGGGCTTGACATTGTCGCACCAGGTGTAAACGTGCAGAGCACATACCCAGGTTCAACGTATGCCAGCTTAAACGGTACATCGATGGCTACTCCTCATGTTGCAGGTGCAGCAGCCCTTGTTAAACAAAAGAACCCATCTTGGTCCAATGTACAAATCCGCAATCATCTAAAGAATACGGCAACGAGCTTAGGAAGCACGAACTTGTATGGAAGCGGACTTGTCAATGCAGAAGCGGCAACACGCTAA |
| Amino Acid Sequence of the Secreted Protein | Length = 380 aa MKKPLGKIVASTALLISVAFSSSIASAAEEAKEKYLIGFNEQEAVSEFVEQVEANDEVAILSEEEEVEIELLHEFETIPVLSVELSPEDVDALELDPAISYIEEDAEVTTMAQSVPWGISRVQAPAAHNRGLTGSGVKVAVLDTGISTHPDLNIRGGASFVPGEPSTQDGNGHGTHVAGTIAALNNSIGVLGVAPSAELYAVKVLGASGSGSVSSIAQGLEWAGNNGMHVANLSLGSPSPSATLEQAVNSATSRGVLVVAASGNSGAGSISYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVNVQSTYPGSTYASLNGTSMATPHVAGAAALVKQKNPSWSNVQIRNHLKNTATSLGSTNLYGSGLVNAEAATR |
| Expression Host | Bacillus subtilis WB600 |
| Source Organism of the Signal Peptide | Bacillus subtilis 168 |
| Source Gene of the Signal Peptide | yndA |
| Signal Peptide Sequence | Length = 26 aa MRFTKVVGFLSVLGLAAVFPLTAQAE |
| Type | Sec |
| Yield | 1777.416 (U/mL) |
| Performance (Current Yield / Highest Yield) | 0.23 |
| Charge | 1 |
| Charge (N-Region) | 2 |
| Hydrophobicity | 1.13 |
| Hydrophobicity Plot |
|
| Hydrophobicity (H-region) | 2.19 |
| Signal Peptide Original Protein Sequence | MRFTKVVGFLSVLGLAAVFPLTAQAEKAGTAGAGEWDKLGTYTYTYSSPTVYSTGGDFRVCLSGSTPFSVSLHLYEDDPGDNPDDYVGANYFSPGECHTFGSIGKFVDGSNNKAEFFVTDYSGKSKTVTFYD |
| Signal Peptide Original DNA Sequence | ATGAGATTCACTAAGGTAGTTGGATTTTTGTCTGTTTTAGGGTTGGCTGCGGTTTTTCCATTAACGGCACAAGCAGAAAAAGCCGGAACTGCGGGGGCTGGAGAGTGGGATAAGTTGGGGACTTACACATATACTTACTCAAGTCCGACGGTATACTCGACCGGAGGAGATTTTAGAGTGTGCCTTTCAGGAAGCACTCCTTTTTCTGTTTCACTGCACTTATATGAGGATGATCCCGGTGACAATCCTGATGATTATGTAGGGGCAAACTATTTTTCACCCGGAGAATGCCACACCTTTGGCAGTATTGGAAAATTTGTAGATGGGAGCAATAACAAGGCCGAGTTCTTTGTTACCGATTACTCTGGTAAATCTAAAACTGTTACGTTTTATGATTGA |
| Reference | Liu Y, Shi C, Li D, et al. Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide[J]. International journal of biological macromolecules, 2019, 138: 903-911. [PubMed] |
| Miscellaneous | - |