SPSED Signal Peptide Secretion Efficiency Database
Detail Information for SPSED0020008
| Accession Number | SPSED0020008 |
| Secreted Protein | Natto phytase |
| Uniprot Accession Number | A0A0H5B9W2 |
| GenBank Accession Number | BAR91210.1 |
| Source Organism of the Secreted Protein | Bacillus subtilis natto starter |
| Nucleotide Sequence of the Secreted Protein | Length = 1363 nt AACAACAAAACTAAATAAACAATGTACTAAATATTCAACATATTTTTTTTTCGCTCCGCAAAAGCAAATCTGGCAAAGTCATCTCACGTACCTCTTTGGGGAATAAGTTGTTTATGAGTCAGAAACCCTATAAAAAAAGGTTCATTTTCCTCACGGTAATTACCTGTATATATTTTATAATAGTAGTGTTAGTGATAAAAGAGGAGGGTACCAAATGAAGGTTTCAAAAACAATGCTGCTAAGCACTGCCGCGGGTTTATTGCTTAGCCTGACAGCAACCTCGGTGTCGGCTCATTATGTGAATGAGGAACATCATTTCAAAGTGACTGCACACACGGAGACAGATCCGGTCGCATCTGGCGATGACGCAGCAGATGACCCGGCCATTTGGGTTCATGAAAAACACCCGGAAAAAAGCAAGTTGATTACAACAAATAAGAAGTCAGGGCTCGTCGTGTATGATTTAGACGGAAAACAGCTTCATTCTTATGAGTTTGGCAAGCTCAATAATGTCGATCTGCGCTATGATTTTCCATTGAACGGCGAAAAAATTGATATTGCTGCCGCATCCAATCGGTCCGAAGGAAAAAATACAATTGAAGTATATGCAATAGACGGGGATAAAGGAAAATTGAAAAGCATTACTGATCCGAACCATCCTATTTCCACCAATATTTCTGAGGTTTATGGATTCAGCTTGTATCACAGCCAGAAAACAGGAGCATTTTACGCATTAGTGACAGGCAAACAAGGGGAATTTGAGCAGTATGAAATTGTTGATGGTGGAAAGGGTTATGTAACAGGGAAAAAAGTGCGTGAATTTAAGTTGAATTCTCAGACCGAAGGCCTTGTTGCGGATGATGAGTACGGAAACCTATACATAGCAGAGGAAGATGAGGCCATCTGGAAATTTAACGCTGAGCCCGGCGGAGGATCAAAGGGGCAGGTTGTTGACCGTGCGACAGGAGATCATTTGACAGCTGATATTGAAGGACTGACAATCTATTATGCACCAAATGGCAAAGGATATCTCATGGCTTCAAGTCAAGGAAATAACAGCTATGCAATGTATGAACGGCAGGGGGAAAATCGCTATGTAGCCAACTTTGAGATTACAGATGGCGAGAAGATAGACGGTACTAGTGACACGGATGGTATTGATGTTCTCGGTTTCGGACTTGGCCCAAAATATCCGTACGGGATTTTTGTGGCGCAGGATGGCGAAAATATCGATAACGGACAAGCCGTCAATCAAAATTTCAAAATTGTATCGTGGGAACAAATTGCACAGCATCTCGGCGAAATGCCTGATCTTCATAAACAGGTGAACCCGAGGAAGCTGAAAGACCGTTCTGACGGCTAG |
| Amino Acid Sequence of the Secreted Protein | Length = 382 aa MKVSKTMLLSTAAGLLLSLTATSVSAHYVNEEHHFKVTAHTETDPVASGDDAADDPAIWVHEKHPEKSKLITTNKKSGLVVYDLDGKQLHSYEFGKLNNVDLRYDFPLNGEKIDIAAASNRSEGKNTIEVYAIDGDKGKLKSITDPNHPISTNISEVYGFSLYHSQKTGAFYALVTGKQGEFEQYEIVDGGKGYVTGKKVREFKLNSQTEGLVADDEYGNLYIAEEDEAIWKFNAEPGGGSKGQVVDRATGDHLTADIEGLTIYYAPNGKGYLMASSQGNNSYAMYERQGENRYVANFEITDGEKIDGTSDTDGIDVLGFGLGPKYPYGIFVAQDGENIDNGQAVNQNFKIVSWEQIAQHLGEMPDLHKQVNPRKLKDRSDG |
| Expression Host | Bacillus subtilis RIK1285 |
| Source Organism of the Signal Peptide | Bacillus subtilis |
| Source Gene of the Signal Peptide | pbpX |
| Signal Peptide Sequence | Length = 39 aa MTSPTRRRTAKRRRRKLNKRGKLLFGLLAVMVCITIWNA |
| Type | Tat |
| Yield | 2570 (μmol/L per min) |
| Performance (Current Yield / Highest Yield) | 0.6 |
| Charge | 12 |
| Charge (N-Region) | 11 |
| Hydrophobicity | -0.37 |
| Hydrophobicity Plot |
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| Hydrophobicity (H-region) | 2.26 |
| Signal Peptide Original Protein Sequence | MTSPTRRRTAKRRRRKLNKRGKLLFGLLAVMVCITIWNALHRNSEENEPSQETAAVSNTDQKKEVKKKTAKKSEEQIKTVDRNQKISNYLKEIGFSGTAMIVRNGEIVTNKGFGYADRKHYIQNNPLTSFYVGSSQKALIATAILQLEEKGKLQTSDPVSTYLPHFPNGQTITLKNLLTHTSGINGHIEGNGAITPDDLIKDIELQGIKRQPGVWDYKDSNYSVLAYIIAEVSGEPYEQYIKNHIFKPAGMTHAGFYKTYEKEPYPAVGYKMEGSKTVTPYIPDLSQLYGAGDIYMSAIDMYKFDQALIDGKLYSQKSYEKMFTPGSSSTYGMGFYVAPGSYSNHGVMPGFNILNSFSKSGQTIVILFSNIQNNAKLGQVNNKIYQLLNQE |
| Signal Peptide Original DNA Sequence | ATGACAAGCCCAACCCGCAGAAGAACTGCGAAACGCAGACGGAGAAAACTAAATAAAAGAGGAAAACTGTTGTTTGGTCTTTTAGCAGTGATGGTTTGCATTACGATTTGGAATGCTCTTCATCGAAATAGTGAAGAAAACGAACCATCTCAAGAAACTGCAGCGGTTTCAAATACCGATCAGAAAAAAGAGGTCAAAAAGAAAACTGCCAAAAAATCAGAGGAACAAATCAAAACAGTGGATCGAAACCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGAACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGCTATGCTGACCGTAAACACTACATTCAAAATAATCCATTGACATCTTTTTATGTCGGTTCATCACAAAAAGCGCTGATTGCAACTGCTATTTTGCAGCTTGAGGAAAAGGGGAAACTTCAGACAAGTGATCCGGTAAGCACGTATTTACCTCATTTTCCCAACGGACAAACGATTACTTTGAAGAATCTGCTTACACATACATCGGGGATAAACGGACATATTGAAGGAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATCCTGCCGTCGGGTATAAGATGGAGGGCAGCAAAACAGTTACACCGTATATACCTGATTTATCCCAGCTGTATGGAGCGGGTGATATTTATATGAGCGCTATAGATATGTACAAATTTGACCAAGCGCTGATAGACGGAAAACTGTATTCGCAAAAAAGCTATGAAAAGATGTTTACGCCAGGGAGCAGCTCCACATACGGCATGGGATTTTATGTTGCTCCCGGAAGCTATTCAAACCATGGTGTTATGCCGGGCTTCAATATCTTGAACAGCTTTAGCAAATCTGGGCAAACCATTGTTATTTTGTTCTCAAACATTCAAAATAATGCTAAATTGGGCCAAGTGAACAATAAAATATACCAGCTTCTAAATCAAGAATGA |
| Reference | Tsuji S, Tanaka K, Takenaka S, et al. Enhanced secretion of natto phytase by Bacillus subtilis[J]. Bioscience Biotechnology and Biochemistry, 2015, 79(11): 1906-1914. [PubMed] |
| Miscellaneous | - |