Accession Number | SPSED0022002 |
Secreted Protein | α-amylase (AmyS) |
Uniprot Accession Number | P06279 |
GenBank Accession Number | AAA22235.2 |
Source Organism of the Secreted Protein | Bacillus stearothermophilus |
Nucleotide Sequence of the Secreted Protein | Length = 1650 nt GTGCTAACGTTTCACCGCATCATTCGAAAAGGATGGATGTTCCTGCTCGCGTTTTTGCTCACTGCCTTGCTGTTCTGCCCAACCGGACAGCCCGCCAAGGCTGCCGCACCGTTTAACGGCACCATGATGCAGTATTTTGAATGGTACTTGCCGGATGATGGCACGTTATGGACCAAAGTGGCCAATGAAGCCAACAACTTATCCAGCCTTGGCATCACCGCTCTTTGGCTGCCGCCCGCTTATAAAGGAACAAGCCGCAGCGACGTAGGGTACGGAGTATACGACTTGTATGACCTCGGTGAATTCAATCAAAAAGGGGCCGTCCGCACAAAATACGGAACAAAAGCTCAATATCTTCAAGCCATTCAAGCCGCCCACGCCGCTGGAATGCAAGTGTACGCCGATGTCGTGTTCGACCATAAAGGCGGCGCCGACGGCACGGAATGGGTGGACGCCGTCGAAGTCAATCCGTCCGACCGCAACCAAGAAATCTCGGGCACCTATCAAATCCAAGCATGGACGAAATTTGATTTTCCCGGGCGGGGCAACACCTACTCCAGCTTTAAGTGGCGCTGGTACCATTTTGATGGCGTTGATTGGGACGAAAGCCGAAAATTGAGCCGCATTTACAAATTCCGCGGCATCGGCAAAGCGTGGGATTGGGAAGTAGACACGGAAAACGGAAACTATGACTACTTAATGTATGCCGACCTTGATATGGATCATCCCGAAGTCGTGACTGAGCTGAAAAGCTGGGGGAAATGGTATGTCAACACAACGAACATTGATGGGTTCCGGCTTGATGCCGTCAAGCATATTAAGTTCAGTTTTTTTCCTGATTGGTTGTCTGATGTGCGTTCTCAGACTGGGAAGCCGCTATTTACCGTTGGGGAATATTGGAGCTATGACATCAACAAGTTGCACAATTACATTATGAAAACAAACGGAACGATGTCTTTGTTTGATGCCCCGTTACACAACAAATTTTATACCGCTTCCAAATCAGGGGGCACATTTGATATGCGCACGTTAATGACCAATACTCTCATGAAAGATCAACCAACATTGGCCGTCACCTTCGTTGATAATCATGACACCGAACCCGGCCAAGCGCTGCAGTCATGGGTCGACCCATGGTTCAAACCGTTGGCTTACGCCTTTATTCTAACTCGGCAGGAAGGATACCCGTGCGTCTTTTATGGTGACTATTATGGCATTCCACAATATAACATTCCTTCGCTGAAAAGCAAAATCGATCCGCTCCTCATCGCGCGCAGGGATTATGCTTACGGAACGCAACATGATTATCTTGATCACTCCGACATCATCGGGTGGACAAGGGAAGGGGTCACTGAAAAACCAGGATCCGGACTGGCCGCATTGATCACCGATGGGCCGGGAGGAAGCAAATGGATGTACGTTGGCAAACAACACGCCGGAAAAGTGTTCTATGACCTTACCGGCAACCGGAGTGACACCGTCACCATCAACAGTGATGGATGGGGGGAATTCAAAGTCAATGGCGGTTCGGTTTCGGTTTGGGTTCCTAGAAAAACGACCGTCTCTACTATCGCTTGGTCGATCACAACCCGACCGTGGACTGATGAATTCGTCCGTTGGACCGAACCACGGTTGGTGGCATGGCCTTGA |
Amino Acid Sequence of the Secreted Protein | Length = 549 aa MLTFHRIIRKGWMFLLAFLLTALLFCPTGQPAKAAAPFNGTMMQYFEWYLPDDGTLWTKVANEANNLSSLGITALWLPPAYKGTSRSDVGYGVYDLYDLGEFNQKGAVRTKYGTKAQYLQAIQAAHAAGMQVYADVVFDHKGGADGTEWVDAVEVNPSDRNQEISGTYQIQAWTKFDFPGRGNTYSSFKWRWYHFDGVDWDESRKLSRIYKFRGIGKAWDWEVDTENGNYDYLMYADLDMDHPEVVTELKSWGKWYVNTTNIDGFRLDAVKHIKFSFFPDWLSDVRSQTGKPLFTVGEYWSYDINKLHNYIMKTNGTMSLFDAPLHNKFYTASKSGGTFDMRTLMTNTLMKDQPTLAVTFVDNHDTEPGQALQSWVDPWFKPLAYAFILTRQEGYPCVFYGDYYGIPQYNIPSLKSKIDPLLIARRDYAYGTQHDYLDHSDIIGWTREGVTEKPGSGLAALITDGPGGSKWMYVGKQHAGKVFYDLTGNRSDTVTINSDGWGEFKVNGGSVSVWVPRKTTVSTIAWSITTRPWTDEFVRWTEPRLVAWP |
Expression Host | Bacillus subtilis RIK1285 |
Source Organism of the Signal Peptide | Bacillus subtilis |
Source Gene of the Signal Peptide | aspB |
Signal Peptide Sequence | Length = 22 aa MKLAKRVSALTPSTTLAITAKA |
Type | Sec |
Yield | 246.1 (U/ml) |
Performance (Current Yield / Highest Yield) | 0.75 |
Charge | 4 |
Charge (N-Region) | 3 |
Hydrophobicity | 0.4 |
Hydrophobicity Plot |
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Hydrophobicity (H-region) | 1.66 |
Signal Peptide Original Protein Sequence | MKLAKRVSALTPSTTLAITAKAKELKAAGHDVIGLGAGEPDFNTPQHIIDAAVRSMNEGHTKYTPSGGLAELKNSIAEKFKRDQNIEYKPSQIIVCTGAKHALYTLFQVILDEEDEVIIPTPYWVSYPEQVKLAGGKPVYVEGLEENHFKISPEQLKNAITEKTKAIVINSPSNPTGVMYTEEELSALGEVCLEHDILIVSDEIYEKLTYGGKKHVSIAQLSDRLKEQTVIINGVSKSHSMTGWRIGYAAGSEDIIKAMTNLASHSTSNPTSIAQYGAIAAYNGPSEPLEEMREAFEHRLNTIYAKLIEIPGFSCVKPEGAFYLFPNAKEAAQSCGFKDVDEFVKALLEEEKVAIVPGSGFGSPENVRLSYATSLDLLEEAIERIKRFVEKHS |
Signal Peptide Original DNA Sequence | TTGAAACTGGCAAAAAGAGTATCCGCATTAACACCATCAACCACACTGGCAATCACAGCGAAAGCGAAAGAACTGAAAGCCGCAGGCCATGATGTCATCGGCTTAGGAGCAGGCGAGCCTGACTTCAATACACCGCAGCATATTATTGATGCCGCTGTGCGTTCGATGAACGAGGGCCATACGAAATACACGCCTTCCGGTGGTTTGGCTGAACTGAAAAACAGCATTGCAGAGAAATTTAAACGCGACCAGAATATTGAATACAAACCGTCTCAAATTATTGTCTGCACTGGTGCCAAACATGCTCTGTATACCCTTTTCCAAGTGATTTTGGATGAAGAAGATGAAGTTATTATTCCAACGCCTTACTGGGTAAGCTACCCGGAGCAGGTGAAACTCGCTGGCGGAAAGCCTGTTTATGTAGAAGGGCTTGAGGAAAATCACTTCAAAATTTCGCCTGAGCAGCTGAAAAATGCAATTACGGAAAAAACAAAAGCAATCGTTATTAACTCTCCAAGCAACCCGACTGGTGTCATGTATACGGAAGAAGAACTATCTGCGCTCGGTGAAGTGTGCCTTGAACATGATATCTTGATTGTGTCAGATGAAATTTATGAAAAACTTACATACGGCGGAAAGAAACATGTTTCCATTGCACAGCTGTCAGACAGACTGAAAGAGCAAACGGTCATTATAAACGGCGTATCGAAGTCCCACAGCATGACAGGCTGGAGAATCGGTTATGCGGCAGGCTCTGAAGACATTATTAAAGCGATGACGAACCTTGCAAGCCACAGCACGTCAAACCCGACATCAATCGCACAATACGGAGCGATTGCTGCTTATAACGGGCCTTCTGAGCCATTGGAAGAAATGAGAGAAGCATTTGAACATAGATTAAATACGATCTATGCAAAGCTCATAGAAATCCCAGGGTTCAGCTGCGTGAAGCCGGAAGGTGCTTTTTACTTGTTCCCAAATGCAAAAGAGGCAGCTCAATCTTGCGGTTTCAAAGATGTAGATGAATTTGTTAAAGCGCTTCTCGAGGAGGAAAAAGTTGCGATTGTTCCAGGATCCGGATTTGGCTCTCCTGAGAATGTCCGTCTTTCTTATGCAACATCGTTAGATCTTCTTGAAGAAGCCATTGAAAGAATCAAGCGTTTTGTAGAAAAACATAGCTAA |
Reference | Ya D, Su L, Li N, et al. Enhanced extracellular expression of Bacillus stearothermophilus alpha-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and alpha-amylase mutant selection[J]. Microbial Cell Factories, 2019, 18: 69. [PubMed] |
Miscellaneous | - |